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Instruments

Flow Cytometry

1. FACS Canto I (6 colours)

  • Measurement of 8 parameters: 6 fluorescence detectors, FSC and SSC
  • 2 lasers: Blue (488nm), Red (635nm)
  • Recommended fluorochromes:
    • FITC and Alexa Fluor 488 (FL1 channel)
    • PE (FL2)
    • PerCP, PerCP-Cy5.5 for FL3 channel
    • PE-Cy-7 for FL4 channel
    • APC or Alexa 647 for FL5 channel
    • APC-H7, APC-Cy7 for FL6 channel

Reserved for measurement of GenTec S1 and S2 classified samples.

2. FACS Calibur (4 colours)

FACS Calibur

  • Measurement of 6 parameters: 4 fluorescence detectors, FSC and SSC
  • 2 Lasers: Blue (488nm), Red (635nm)
  • Recommended fluorochromes:
    • FITC and Alexa Fluor 488 (FL1 channel)
    • PE (FL2)
    • PerCP, PE-Cy5, PerCP-Cy5.5 and PE-Cy7 for FL3 channel
    • APC and Alexa 647 for FL4

Please note that Pe-Cy5 and APC should not be used simultaneously on FACS Calibur. Reserved for measurement of fluorescence-labelled bacteria, GenTec S1.

3. FACS Canto II (8 colors)

  • Measurement of 10 parameters: 8 fluorescence detectors, FSC and SSC
  • 3 lasers: Violett (405nm), Blue (488nm), Red (635nm)
  • Recommended fluorochromes:
    • FITC and Alexa Fluor 488 (FL1 channel)
    • PE (FL2)
    • PerCP, PerCP-Cy5.5 for FL3 channel
    • PE-Cy-7 for FL4 channel
    • APC or Alexa 647 for FL5 channel
    • APC-H7, APC-Cy7 for FL6 channel
    • BV421 for FL7 channel
    • BV510 for FL8 channel

Reserved for measurement of GenTec S1 and S2 classified samples.

Cytometry based cell sorter

FACS ARIA III (8 colours)

  • Measurement of 9 parameters: 7 fluorescence detectors, FSC and SSC
  • 2 lasers: Blue (488nm), Red (633nm)
  • Sorting into different tubes, well plates and onto microscope slides
  • Sorting of GenTec S1 and S2 classified samples is possible.
  • Recommended fluorochromes:
    • FITC and Alexa Fluor 488 (FL1 channel)
    • PE (FL2)
    • PerCP, PerCP-Cy5.5 for FL3 channel
    • PE-Cy-7 for FL4 channel
    • APC or Alexa 647 for FL5 channel
    • APC-H7, APC-Cy7 for FL6 channel
    • Alexa 750 for FL7 channel

Magnetic cell sorting (cell sort according to surface antigens):

AutoMACS Pro (program-based cell sorting, specialized sorts for whole blood specimens)

  • MACS Microbead Technology for magnetic cell sorting
  • Walk-away cell isolation with automated labeling and separation
  • Fast and gentle isolation of virtually any cell type from any species
  • Sorting of GenTec S1 and S2 classified samples is possible.

Tissue dissoziation

OctoMACS (dissoziation of human and animal tissues into single cells for downstream applications)

The OctoMACS  Dissociator with Heaters is equipped with several programs for fully automated tissue dissociation into single-cell suspensions, including:

  • Mouse or human tumor
  • Mouse or rat neonatal heart
  • Neural tissue
  • Mouse spleen, lung, lamina propria, epidermis, or liver
  • Mouse or rat skeletal muscle  

Contact

Dr. Gunter Rappl CMMC Cologne
Dr. Gunter Rappl

Center for Molecular Medicine Cologne | Cell Sorting Facility

Operator - Cell Sorting Facility

+49 221 478 89850

+49 221 478 88723

Center for Molecular Medicine Cologne | Cell Sorting Facility

Robert-Koch-Straße 21

50931 Cologne

Registration | Support

Contact Facility staff, CMMC Research Building 2nd floor (room 2.033), preferentially by email. Your request will be scheduled as soon as possible.

We are strictly following the Road Map for Cell Sort Service to ensure high quality service in due time.

Support

First Tuesday of each month at 1:00 p.m. via Zoom

Discover more:

 
University of Cologne (Universtität zu Köln)
Faculty of Medicine Faculty of Mathematics
and Natural Sciences
University Hospital of Cologne (Universitätsklinik zu Köln)

Copyright ©
Prof. Dr. med. Thomas Benzing
Chair of the CMMC