Center for Molecular Medicine Cologne

Kurt P Pfannkuche - assoc. RG 17

Cardiac tissue engineering

Abstract

Our lab focusses on induced pluripotent stem cell derived cardiomyocytes. We aim to optimize transplantation of micro-tissues into the myocardium after myocardial infarction. Micro-tissues refer to aggregates of organotypic cells with defined size and cellular composition. Major obstacles to be solved include the occurrence of cardiac arrhythmia after cell transplantation into the diseased myocardium, the low efficiency of cardiomyocyte engraftment and the technological deficits in cell preparation.

Current projects of the group address questions of cell engraftment in different animal models. Improved bioreactor technologies and 3D culture models are being developed. Today it remains unclear which strategies will result in a stem cell-based therapy of cardiac diseases in the future and we follow additional strategies beside direct cell transplantation.

We are interested in developing artificial cardiac tissues that are generated from pluripotent stem cell derived cells. In this respect, formation of artificial vessel structures to provide oxygen and nutrients to the artificial myocardium is part of ongoing research activities. During 2020-2023 the group will develop a novel model of organ chimaeras to study the process of cardiac organ complementation.

Finally, we are addressing mechanism of cardiac fibrosis with focus on the role of cardiomyocytes in cardiac matrix homeostasis.

Clinical and Medical Relevance

Cardiovascular diseases are among the major causes of death in the western hemisphere.

Stem cell based technologies will pave the way towards novel therapies of cardiac diseases such as cardiac infarction.

Our research strategy will generate knowledge in the field of artificial tissues and organs for future therapies in vivo as well as novel test systems for pharmaceuticals in vitro.

Project Related Publications

  1. Hamad S, Derichsweiler D, Papadopoulos S, Nguemo F, Šarić T, Sachinidis A,Brockmeier K, Hescheler J, Boukens BJ, Pfannkuche K. Generation of human induced pluripotent stem cell-derived cardiomyocytes in 2D monolayer and scalable 3D suspension bioreactor cultures with reduced batch-to-batch variations. (2019).Theranostics. 9(24), 7222-7238.
  2. Sahito RGA, Sheng X, Maass M, Mikhael N, Hamad S, Heras-Bautista CO,Derichsweiler D, Spitkovsky D, Suhr F, Khalil M, Brockmeier K, Halbach M, Saric T, Hescheler J, Krausgrill B, Pfannkuche K. (2019). In Vitro Grown Micro-Tissues for Cardiac Cell Replacement Therapy in Vivo. Cell Physiol Biochem. 52(6), 1309-1324.
  3. Heras-Bautista CO, Mikhael N, Lam J, Shinde V, Katsen-Globa A, Dieluweit S, Molcanyi M, Uvarov V, Jütten P, Sahito RGA, Mederos-Henry F, Piechot A, Brockmeier K, Hescheler J, Sachinidis A, Pfannkuche K. Cardiomyocytes facing fibrotic conditions re-express extracellular matrix transcripts. (2019). Acta Biomater. 89, 180-192.
  4. Heras-Bautista CO, Katsen-Globa A, Schloerer NE, Dieluweit S, Abd El Aziz OM, Peinkofer G, Attia WA, Khalil M, Brockmeier K, Hescheler J, Pfannkuche K. The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes. (2014). Biomaterials 35(26), 7374-85.
  5. Sheng X, Reppel M, Nguemo F, Mohammad FI, Kuzmenkin A, Hescheler J, Pfannkuche K. Human pluripotent stem cell-derived cardiomyocytes: response to TTX and lidocain reveals strong cell to cell variability. (2012). PLoS One. 7(9):e45963.
Dr. Kurt P Pfannkuche CMMC Cologne
Dr. Kurt P Pfannkuche

Institute of Neurophysiology

CMMC - assoc. Research Group

+49 221 478 6940

Institute of Neurophysiology

Robert-Koch Str. 39

50931 Cologne

CMMC Profile Page

Curriculum Vitae (CV)

Publications on PubMed

Publications - Kurt P Pfannekuche

Link to PubMed

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