Pancreatic ductal adenocarcinoma (PDAC) is amongst the deadliest cancers with a 5-year survival rate of below 5%. This poor prognosis is caused by absence of specific early symptoms and primary resistance to chemotherapy highlighting an urgent need to address these two problems. Molecularly, PDAC is characterised by the presence of activating point mutation in the small GTPase KRAS in 95% of the cases. This point mutation leads to constitutive activation of KRAS, promoting proliferation, invasion and cell death resistance. We recently contributed to a study identifying that the cystine/glutamate antiporter xCT (SLC7A11) promotes Ras-mediated transformation by protecting cells from reactive oxygen species (ROS) overload (Lim et al., 2019). Interestingly, xCT has been implicated in the protection against a recently discovered form of regulated necrosis, termed ferroptosis. Sensitivity to ferroptosis, in turn, has emerged as a selective vulnerability in cancer cells which escape targeted treatment. Based on these findings, we have experimentally explored whether the KRASG12D mutation which is prevalent in PDAC might be promoting malignancy through evading ferroptosis. The aim of this project to establish i) whether ferroptosis evasion might determine KRASG12D fitness in PDAC development ii) and how breaking ferroptosis evasion might affect the development of PDAC precursor lesions in a genetically-engineered mouse model. We thereby anticipate to identify cell death vulnerabilities in conjunction with potential markers during early PDAC progression for the development of novel treatment strategies.
The poor 5-year survival rate of below 5% of PDAC patients has not significantly improved since the 1970ies. This has marked PDAC as one of the cancers with unmet needs warranting the urgent requirement to explore novel therapeutic strategies. We propose to test ferroptosis-inducing therapy (FIT) in in-vitro and in-vivo PDAC model systems in order to address this need. Findings made in these model systems will be corroborated in a PDAC patient tissue bank.
Most tumours are known to undergo constant cycles of selection via immune effector cells. One pathway via which immune effector cells kill target cells is extrinsic apoptosis induction via ligand/receptor binding of Tumor necrosis factor (TNF)- and TNF-receptor (TNFR) superfamily (SF) members (Karstedt et al., 2017). Yet, given the fast kinetics and efficiency by which extrinsic apoptosis can eliminate targeted cells, it is not surprising that tumours frequently develop escape mechanisms against extrinsic apoptosis. In KRAS-driven PDAC, we previously found that extrinsic apoptosis is disabled (Karstedt et al., 2015). Interestingly, Ferroptosis is a recently discovered type of regulated necrosis which, unlike apoptosis, is independent of caspase. Instead, ferroptotic cells die following iron-dependent lipid peroxidation, a process which is antagonised by glutathione peroxidase 4 (GPX4), by the cystine/glutamate antiporter xCT (SLC7A11) and ferroptosis suppressor protein 1 (FSP1) (reviewed in Bebber et al. under review). Importantly, tumour cells escaping other forms of regulated cell death maintain or acquire sensitivity to ferroptosis (Thomas*, Bebber* et al. in preparation). As part of this project, it will be tested whether KRAS-mutated PDAC which is resistant to extrinsic apoptosis can respond to ferroptosis-inducing therapy (FIT). Findings made unbiased studies will be validated mechanistically and tested in genetically engineered mouse models for PDAC.
Department of Translational Genomics - CECAD Research Center
CMMC - PI - A 06
silvia.von-karstedt2[at]uk-koeln.de
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Department of Translational Genomics - CECAD Research Center
Joseph-Stelzmann-Str. 26
50931 Cologne
CECAD Cologne
CRC 1399: Mechanism of Drug Sensisitvity and resistance in Small Cell Lung Cancer
CRC 1403: Cell Death in Immunity, Inflammation and Disease
Ariadne Androulidaki, PostDoc
Eric Seidel, PostDoc
Erica Benard, PostDoc
Christina Bebber, PhD Student
Fabienne Müller, PhD Student
Madhuri Puvvada, PhD Student
Sofya Tishina, PhD Student
Fatma Isil Yapici, PhD Student
Jenny Stroh, Technician