Center for Molecular Medicine Cologne

Hisham Bazzi - assoc. RG 02

The molecular pathways regulating skin morphogenesis and regeneration

Abstract

Cell division and cell migration have been extensively studied in cell lines in vitro; however, how these processes are regulated in vivo in physiological and pathological contexts remain poorly defined. In particular, the upstream signalling pathways dictating cell migration and cell fates are not well characterized. Our aim is to use the developing and regenerating mouse skin to dissect the molecular pathways of keratinocyte and fibroblast division and migration. We have recently performed scRNAseq analyses of developing skin. We are utilizing this rich atlas of gene expression changes in the different cell populations of the skin, including the specialized hair follicle forming cells in the epithelium and mesenchyme, to guide our candidate gene and pathway analyses. We have also generated new mouse lines using cutting-edge CRISPR-Cas9 technology. Our work will have profound implications for understanding how cell division and migration are regulated in the mouse in vivo, which will aid in developing better therapies for skin pathologies that depend on these processes such as wound healing and cancer metastasis.

Clinical and Medical Relevance

How cell division and migration are regulated during skin morphogenesis and regeneration, as well as in pathological conditions such as wound healing are poorly understood.

Using genetic approaches in the mouse skin and candidate genes from our scRNAseq data, we are dissecting the molecular pathways governing these processes in the mouse in vivo.

Our study will help develop clinical therapies for skin pathologies that recapitulate developmental programs.

Project Related Publications

  1. Xiao, C., Nitsche, F., Bazzi, H. (2018). Visualizing the node and notochordal plate in gastrulating mouse embryos using scanning electron microscopy and whole mount immunofluorescence. J Vis Exp 141.
  2. Bazzi, H.#, Soroka, E., Alcorn, H., Anderson, K.V.#. (2017). STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo. Proc Natl Acad Sci U S A 114(51), E10928-E10936. # Co-correspondence.
  3. Insolera, R.*, Bazzi, H.*, Shao, W., Shi, S.H., Anderson, K.V. (2014). Cortical neurogenesis in the absence of centrioles. Nat Neurosci 17(11), 1528-35. * Co-first authorship.
  4. Bazzi, H., Anderson, K.V. (2014). Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo. Proc Natl Acad Sci U S A 111 (15), E1491-500.
  5. Bazzi, H., Demehri, S., Potter, C.S., Barber, A.G., Awgulewitsch, A., Kopan, R., Christiano, A.M. (2009). Desmoglein 4 is regulated by transcription factors implicated in hair shaft differentiation. Differentiation 78(5), 292-300.
  6. Bazzi, H., Fantauzzo, K.A., Richardson, G.D., Jahoda, C.A., Christiano, A.M. (2007). The Wnt inhibitor, Dickkopf 4, is induced by canonical Wnt signalling during ectodermal appendage morphogenesis. Dev Biol 305(2), 498-507.
  7. Bazzi, H., Fantauzzo, K.A., Richardson, G.D., Jahoda, C.A., Christiano, A.M. (2007). Transcriptional profiling of developing mouse epidermis reveals novel patterns of coordinated gene expression. Dev Dyn 236(4), 961-70.
  8. Kljuic, A., Bazzi, H., Sundberg, J.P., Martinez-Mir, A., O'Shaughnessy, R., Mahoney, M.G., Levy, M., Montagutelli, X., Ahmad, W., Aita, V.M., Gordon, D., Uitto, J., Whiting, D., Ott, J., Fischer, S., Gilliam, T.C., Jahoda, C.A., Morris, R.J., Panteleyev, A.A., Nguyen, V.T., Christiano, A.M. (2003). Desmoglein 4 in hair follicle differentiation and epidermal adhesion: evidence from inherited hypotrichosis and acquired pemphigus vulgaris. Cell 113(2), 249-60.
Dr. Hisham Bazzi CMMC Cologne
Dr. Hisham Bazzi

Department of Dermatology / RG location - CECAD Building

CMMC - assoc. Research Group

Department of Dermatology / RG location - CECAD Building

Joseph-Stelzmann Strasse 26

50931 Cologne

CMMC Profile Page

Publications on PubMed

Publications - Hishami Bazzi

Link to PubMed

Group Members

Ekaterina Soroka
Houda Khatif
Marta Grzonka
Charlotte Gerards
Miriam Mack

Figure 1